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Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies Electron. J. Biotechnol.
Abdullah,Ruslan; Zainal,Alizah; Yew Heng,Wee; Chui Li,Leaw; Chee Beng,Yeap; Mei Phing,Lee; Abdullah Sirajuddin,Salwa; Soo Ping,Winnie Yap; Lourdes Joseph,Juanita; Azma Jusoh,Siti.
Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation...
Tipo: Journal article Palavras-chave: Genetic transformation studies; Immature embryos; In vitro culture; Oil palm; Plant regeneration.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006
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Effect of Brachionus rubens on the growth characteristics of various species of microalgae Electron. J. Biotechnol.
Abou,Reda A.I; Singh,Manjinder; Rivera-Cruz,Anangelica; Power,Grace; Bagby-Moon,Thomas; Das,Keshav.
Background: Cultivation of algae for conversion to biofuels has gained global interest. Outdoor raceway cultivation is preferred because of its lower capital and operating costs. A major disadvantage of outdoor cultivation is susceptibility of algal crops to attack by predatory rotifers. In order to quantify the impact of rotifer attack on different species of algae, we evaluated the growth of eleven microalgal species over a 21-d period after being infected by the predatory rotifer Brachionus rubens. Results: Of the eleven species, Chlorella sorokiniana was the most susceptible with rapid decline in algal growth concomitant with increase in rotifer population growth (3.82/d). In contrast, Synechococcus elongatus andScenedesmus dimorphus were both...
Tipo: Journal article Palavras-chave: Algae; Biofuels; Biomass; Cyanobacteria; FlowCam; Grazer.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400010
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Measuring b-Galactosidase activity at pH 6 with a differential pH sensor Electron. J. Biotechnol.
Acevedo,Cristian; Stach,Matthias H; Amtmann,Anette; Young,Manuel E; Reyes,Juan G; Huebner,Holger; Buchholz,Rainer.
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
Tipo: Journal article Palavras-chave: B-Galactosidase; Delta milli pH; Differential pH.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200012
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Present and future of bioleaching in developing countries Electron. J. Biotechnol.
Acevedo,Fernando.
Nowadays bioleaching occupies an increasingly important place among the available mining technologies. Today bioleaching is no longer a promising technology but an actual economical alternative for treating specific mineral ores. An important number of the current large-scale bioleaching operations are located in developing countries. This situation is determined by the fact that several developing countries have significant mineral reserves and by the characteristics of bioleaching that makes this technique especially suitable for these countries because of its simplicity and low capital cost requirement. The current situation of commercial-size bioleaching operations and ongoing projects in developing countries is presented and discussed with especial...
Tipo: Journal article
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200001
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The use of reactors in biomining processes Electron. J. Biotechnol.
Acevedo,Fernando.
Microbial processes applied to mining operations are gaining increasing interest in the last years. Potential and current applications include the mining of gold, copper and other heavy metals, desulfurization of coal and oil, tertiary recovery of oil and biosorption of metal ions. Currently, bacterial leaching of copper and biooxidation of refractory gold concentrates are well-established large-scale processes that are carried on using heaps and tank reactors. Heap operation is simple and adequate to handle large volumes of minerals, but their productivity and yields are limited because of the severe difficulties in exerting an adequate process control. On the other hand, reactors can economically handle moderate volumes of material, but they allow for a...
Tipo: Journal article
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300002
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Problem solving in enzyme biocatalysis Electron. J. Biotechnol.
Acevedo,Fernando.
Tipo: Journal article
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400001
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Globalisation, a new paradigm Electron. J. Biotechnol.
Acevedo,Fernando.
Tipo: Journal article
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100001
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Microsatellite markers in candidate genes for wood properties and its application in functional diversity assessment in Eucalyptus globulus Electron. J. Biotechnol.
Acuña,Cintia V; Villalba,Pamela V; García,Martín; Pathauer,Pablo; Hopp,H. Esteban; Marcucci Poltri,Susana N.
Background: Functional genetic markers have important implications for genetic analysis by providing direct estimation of functional diversity. Although high throughput sequencing techniques for functional diversity analysis are being developed nowadays, the use of already well established variable markers present in candidate genes is still an interesting alternative for mapping purposes and functional diversity studies. SSR markers are routinely used in most plant and animal breeding programs for many species including Eucalyptus. SSR markers derived from candidate genes (SSR-CG) can be used effectively in co-segregation studies and marker-assisted diversity management. Results: In the present study, eight new non reported SSRs were identified in seven...
Tipo: Journal article Palavras-chave: Functional markers; Genetic diversity; Lignin pathway; SSR; Wood density.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200002
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Segregation distortion in homozygous lines obtained via anther culture and maize doubled haploid methods in comparison to single seed descent in wheat (Triticum aestivum L.) Electron. J. Biotechnol.
Adamski,Tadeusz; Krystkowiak,Karolina; Kuczynska,Anetta; Mikolajczak,Krzysztof; Ogrodowicz,Piotr; Ponitka,Aleksandra; Surma,Maria; Slusarkiewicz-Jarzina,Aurelia.
Background The quality of wheat grain depends on several characteristics, among which the composition of high molecular weight glutenin subunits, encoded by Glu-1 loci, are the most important. Application of biotechnological tools to accelerate the attainment of homozygous lines may influence the proportion of segregated genotypes. The objective was to determine, whether the selection pressure generated by the methods based on in vitro cultures, may cause a loss of genotypes with desirable Glu-1 alleles. Results Homozygous lines were derived from six winter wheat crosses by pollination with maize (DH-MP), anther culture (DH-AC) and single seed descent (SSD) technique. Androgenetically-derived plants that originated from the same callus were examined before...
Tipo: Journal article Palavras-chave: Androgenesis; DH lines; HMW glutenin subunits; SSD technique; Winter wheat.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100002
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EDITORIAL ELECTRONIC JOURNAL OF BIOTECHNOLOGY ....MOVING FROM SCIENCE TO DEVELOPMENT...: The need to strength collaborative teams in lung cancer research Electron. J. Biotechnol.
Adonis,Marta; Gil,Lionel.
Tipo: Journal article
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200001
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Sequential use of ammonium and leucine as nitrogen sources during growth of Geotrichum candidum on a glucose based medium Electron. J. Biotechnol.
Adour,Lydia; Bude,Florence; Amrane,Abdeltif.
Geotrichum candidum growth on ammonium and leucine as nitrogen sources and glucose as a carbon source was examined. A clear preference of G. candidum for ammonium over leucine as a nitrogen source was shown. Indeed, ammonium was completely exhausted at the end of exponential growth after less than 35 hrs of culture; in contrast only 5% of leucine was concomitantly assimilated. Growth continued at slower rates on glucose and leucine as carbon and nitrogen sources respectively, and at the end of culture (185 hrs), leucine was completely exhausted.
Tipo: Journal article Palavras-chave: Amino acids; Batch culture; Diauxic growth; Geotrichum candidum; Growth kinetics.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100013
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Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier Electron. J. Biotechnol.
Afaq,Sarah; Iqbal,Jawaid.
A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity.
Tipo: Journal article
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300006
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Biosorption of chromium (VI) from aqueous solutions by the husk of Bengal gram (Cicer arientinum) Electron. J. Biotechnol.
Ahalya,N; Kanamadi,R.D; Ramachandra,T.V.
The potential to remove Cr (VI) from aqueous solutions through biosorption using the husk of Bengal gram (Cicer arientinum), was investigated in batch experiments. The results showed removal of 99.9% of chromium in the 10 mgl-1 chromium solution, the biomass required at saturation was 1 g mg-1. Kinetic experiments revealed that the dilute chromium solutions reached equilibrium within 180 min. The biosorptive capacity of the (bgh) was dependent on the pH of the chromium solution, with pH 2 being optimal. The adsorption data fit well with the Langmuir and Freundlich isotherm models. The adsorption capacity calculated from the Langmuir isotherm was 91.64 mg Cr (VI)/g at pH 2. The adsorption capacity increased with increase in agitation speed and an optimum...
Tipo: Journal article Palavras-chave: Agro-wastes; Bengal gram husk (bgh); Cicer arientinum; Chromium biosorption; Isotherms; Fourier transform infrared spectroscopy (FTIR).
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300005
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Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE Electron. J. Biotechnol.
Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001
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Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum Electron. J. Biotechnol.
Ahmed,Sibtain; Imdad,Syeda Sana; Jamil,Amer.
Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T....
Tipo: Journal article Palavras-chave: C. thermophilum; Kinetics; T. harzianum; Xylanase.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003
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Production of natural and rare pentoses using microorganisms and their enzymes Electron. J. Biotechnol.
Ahmed,Zakaria.
Biochemical methods, usually microbial or enzymatic, are suitable for the production of unnatural or rare monosaccharides. D-Arabitol was produced from D-glucose by fermentation with Candida famata R28. D-xylulose can also be produced from D-arabitol using Acetobacter aceti IFO 3281 and D-lyxose was produced enzymatically from D-xylulose using L-ribose isomerase (L-RI). Ribitol was oxidized to L-ribulose by microbial bioconversion with Acetobacter aceti IFO 3281; L-ribulose was epimerized to L-xylulose by the enzyme D-tagatose 3-epimerase and L-lyxose was produced by isomerization of L-RI. L-ribose and L-arabinose were prepared biochemically from ribitol by oxidation using Acetobacter aceti IFO 3281 and isomerization using L-RI and L-arabinose isomerase...
Tipo: Journal article
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200008
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Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method Electron. J. Biotechnol.
Akritidis,Paschalis; Pasentsis,Konstantinos; Tsaftaris,Athanasios S; Mylona,Photini V; Polidoros,Alexios N.
Entering the second decade of commercialization of biotech crops, the global area cultivated with transgenic plants constantly expands and national legislations in many countries, particularly in the European Union, require identification and labeling of genetically modified material in food and feed. We describe here a procedure for characterizing transgenic material of unknown origin present in conventional seed lots using a genome walking strategy for isolation and characterization of the junction between the inserted transgene construct and the host plant genomic DNA. The procedure was applied to transgenic cotton detected as adventitious or technically unavoidable presence in a conventional commercial cultivar. The structure of the isolated region...
Tipo: Journal article Palavras-chave: Event specific detection; Genetically modified cotton; Genome walking; GMO detection; Mon1445.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200010
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Relationship between cultivation mode of white rot fungi and their efficiency for olive oil mill wastewaters treatment Electron. J. Biotechnol.
Alaoui,Sanae Mdaghri; Merzouki,Mohamed; Penninckx,Michel J; Benlemlih,Mohamed.
Four white rot fungi (WRF) strains, Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona and Pycnoporus coccineus, were tested for efficiency of treatment of Olive Oil mill wastewaters (OOMW) in relation with their cultivation mode, i.e. under the form of free mycelium, mycelium immobilized in alginate beads and solid state cultivation on Petri dishes. Study of biodegradation of phenolic compounds, chemical oxygen demand (COD) decrease and decolourisation of OOMW have shown that Coriolopsis polyzona and Pycnoporus coccineus degradation performances were apparently only slightly affected by the cell cultivation procedures experienced here. In contrast, Phanerochaete chrysosporium and Trametes versicolor showed respectively marked...
Tipo: Journal article Palavras-chave: Biotreatment; Cell immobilization; Olive oil mill wastewaters; White rot fungi.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400013
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Resistance of diethylenetriaminepentaacetic acid to anaerobic biodegradation Electron. J. Biotechnol.
Alarcón,Evelyn; Decap,Jacqueline; Vidal,Gladys.
Kraft mills are responsible for the massive discharge of highly polluted effluents, and new bleaching processes (i.e. Total Chlorine Free (TCF)) is presented as a feasible option to reduce this environmental impact. However, increased TCF pulp production is accompanied by an increase in chelate use. The most commonly used chelates, ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DPTA), are considered to be relatively persistent substances in water treatment plants, and consequently environmentally critical compounds. The purpose of this work is to investigate DPTA behaviour in an anaerobic system. An Anaerobic Filter (AF) was operated with three different DPTA load rates (LR DPTA = 0.07 - 0.28 gDPTA/L×d), and the...
Tipo: Journal article Palavras-chave: Anaerobic biodegradation; DTPA; Kraft mill effluent.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300011
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The power of 28 microsatellite markers for parentage testing in sheep Electron. J. Biotechnol.
Al-Atiyat,Raed M.
Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common problem of significant error in pedigree records in Merino sheep. Three different flocks of Australian Merino sheep were investigated. A private farm flock represents a flock with no record availability. Two other flocks were maintained under good managements of full keeping records and being selected for high and low parasite resistances. Results In the studied panel, eight MSTs provided an average of Polymorphic Information content (PIC) equal to 0.65 or more in...
Tipo: Journal article Palavras-chave: Merino sheep; Paternity verification; Pedigree records; SSR markers.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200009
Registros recuperados: 978
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